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Proteintech
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R&D Systems
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Boster Bio
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Becton Dickinson
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GeneTex
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Becton Dickinson
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Miltenyi Biotec
helios antibody, anti-human/mouse ![]() Helios Antibody, Anti Human/Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/anti+helios/Helios+Antibody%2C+anti-human%2Fmouse/custom%40130-104-032%4042382741 Average 92 stars, based on 1 article reviews
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Rabbit anti-Human IKZF2 Polyclonal Antibody
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PerCP/Cy5.5 anti-mouse/human Helios [22F6]; Isotype: Armenian Hamster IgG; Reactivity: Mouse, Human; Apps: ICFC; Size: 25 tests
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Image Search Results
Journal: ImmunoHorizons
Article Title: Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells
doi: 10.4049/immunohorizons.1700064
Figure Lengend Snippet: ( A ) CD4SP GFP + cells inclusive of doublets were sorted from Foxp3 gfp thymocytes. Cells were stained with DAPI for DNA, anti–β-tubulin Ab, anti-Foxp3 Ab, and anti-CD25 Ab. Three representative sibling pairs with asymmetric Foxp3 expression and concordant asymmetry of CD25 abundance. Scale bar, 5 μm. ( B ) Top, Quantification of CD25 asymmetry in cytokinetic pairs with asymmetric Foxp3 expression and at least one CD25 + sibling ( n = 18). Dotted line denotes asymmetry value of 0.2, where values >0.2 are considered asymmetric. Bottom, Among sibling pairs with asymmetric Foxp3 and detectable CD25 signal, 17 out of 18 pairs had concordant asymmetry of CD25 (94%); one sibling pair had symmetrical CD25. *** p < 0.0001 compared with DNA. ( C ) CD4SP CD25 + cells inclusive of doublets were sorted from Foxp3 gfp thymocytes. Cells were stained with DAPI for DNA, anti–β-tubulin Ab, anti-Foxp3 Ab, and anti-Helios Ab. Among sibling pairs with discordant Foxp3 expression, representative images wherein Foxp3 − sister was Helios + (upper rows) or Helios − (lower rows). ( D ) Top, Quantification of Helios abundance in the Foxp3 − sister of Foxp3-discordant sibling pairs ( n = 26). Values greater and lower than 20,000 are considered positive and negative, respectively. Bottom, Among Foxp3-discordant sibling pairs, pie chart summarizing frequency of Foxp3 − sisters that were Helios + (54%) or Helios − (46%).
Article Snippet: Abs include the following: rat anti–β-tubulin (YOL1/34; Abcam), rabbit anti-Foxp3 (C29H4; Cell Signaling Technology), mouse anti-GFP CF 488 (9F9.F9; Sigma-Aldrich), rat anti-CD25 (PC61; BioLegend),
Techniques: Staining, Expressing